Evaluation of magnetic nanoparticles influence on hyaluronic acid production from Streptococcus equi

Abstract This work aims at developing a novel hyaluronic acid (HA) production method using magnetic nanoparticles (NPs). In a separate process, HA was produced with the addition of the amino acids (AA) as bio-additives. Regarding the NPs additives, the results showed that the highest dry weight of the produced HA was 0.264 g/l with the addition of 20 mg/l of Fe 3 O 4 NPs. Concerning the AA additives, the results showed that the highest dry weight of the produced HA was 0.079 g/l with the addition of 0.26 g/l of glutamic acid (GA) compared to the control produced 0.065 g/l. These results led to further develop a novel HA production method which is preparing the Fe 3 O 4 NPs using GA as stabilizer, where the results showed that dry weight of the produced HA was 0.435 g/l with the addition of 20 mg/l of Fe 3 O 4 -GA NPs.