Gas Liquid Chromatography of Trichothecene Mycotoxins

In this paper, a gas liquid chromatography for detection and determination of trichothecenes which were toxic metabolites of Fusarium sp. was described.Trimethyl silylation, acethylation and trifluoroacethylation of trichothecenes were studied. From these results, it was found that trimethylsilyl ether was more suitable than acetate or trifluoroacetate for gas liquid chromatography. Trimethyl silylation of nivalenol and the related compounds (fusarenon and diacethylnivalenol) was difficult. They were imperfectly silylated by bis-trimethylsilyl acetamide, hexamethyl disilazane+trimethyl chlorosilane or N-trimethylsilyl imidazole. However using a mixture of N-trimethylsilyl imidazole, trimethyl chlorosilane and pyridine as silylating reagent they were easily and completely converted to trimethylsilyl ethers.SE-30 as well as OV-17 was the most suitable liquid phase for detection and determination for trimethylsilyl ethers of trichothecenes by gas liquid chromatography, but another phases such as QF-1, XE-60 and NPGA were also useful.