Detection of HIV coreceptor CCR5 mutation in uigur population from sinkiang uigur autonomous region

Whole blood samples were collected from 316 Uigur subjects inhabiting Kashi City, Sinkiang Uigur Autonomous Region, and their genomic DNA was extracted using QIAgen Blood Kit. DNA fragment spanning CCR5#triangle open#32 and CCR5m303 mutation points were amplified by PCR and genotyped by directed electrophoresis and RFLP with HinCII respectively. In all 316 samples only 22 heterozygotes of CCR5#triangle open#32 were found, in accordance with Hardy-Weinberg equilirium. No CCR5m303 mutation was found. No difference existed between male and female individuals. The frequency of CCR5#triangle open#32 in Uigur population is 3.48%, similar to Medi-Asia groups (higher than that of Chinese Han people and lower than that of Caucasian people), i. e, in continuity regarding district distribution. Due to its slow-down effect on clinical course of AIDS, high frequency of CCR5#triangle open#32 is highly significant in prevention and treatment of AIDS in Uigur population.