Generation of Highly Diverse Peptide Library by Linear-double-stranded DNA Based AND Logic Computation in Mammalian Cells

Our previous work established linear-double-stranded DNA (ldsDNA) as innovative biological parts for building low noise-signal ratio AND gate circuits in mammalian cells. Here by introducing NNK degenerate codon nucleotides to ldsDNA terminals, we developed a novel methodology for building highly diverse peptide library. Deep sequencing results revealed that our novel method could generate peptide library with both amino acid sequence and peptide length diversities. Here for the first time, our work established ldsDNA as biological parts for building highly diverse peptide library in mammalian cells, which showed great application potential in generating antigen-receptor (antibodies and T-cell receptors) repertoire and functional peptide libraries.