Образование долгоживущих радикалов белков сыворотки крови млекопитающих при воздействии лазерного излучения и тепла

It has been previously shown that reactive oxygen species (ROS) produce the long-lived reactive protein species (LRPS) as a result of ionizing radiation exposure. Since laser radiation and thermal effects in water solutions also result in ROS generation, this study was aimed to investigate and demonstrate the possibility of LRPS formation from the blood serum proteins – albumin and gamma-globulin – in response to moderate laser radiation and hyperthermia, as well as the production of ROS. Chemiluminescence of BSA and gamma-globulin (GG) in solutions subjected to moderate heating allowed detecting LRPS with a half-life of about 4 h, while enhanced chemiluminescence using the luminol para-iodophenol peroxidase system testified prolonged generation of Н2О2 in BSA and GG solutions. The obtained data indicate the ability of blood serum proteins – albumins and globulins – to form LRPS upon laser radiation and heating. They can act as components of an antioxidant protection system required for inactivation of singlet oxygen, also playing a signaling and regulatory role in the processes related to Н2О2 formation.