VIP-Helodermin receptors in the murine virus-induced T lymphoma cell line BL/VL3 recover less rapidly than β-adrenoceptors after down regulation

AbstractThe time course of recovery of β-adrenergic and VIP/helodermin receptors after homologous and heterologous down regulation was studied in the murine lymphoma cell line BL/VL3, a neoplastic equivalent of immature T cells.The heterologous part of isoproterenol down regulation was rapidly reversed, even in the presence of cycloheximide, suggesting that down regulation was linked to ligand-receptor interaction and/or cyclic AMP increase.Homologous down regulations of β-adrenoceptors and VIP/helodermin receptors were less rapidly reversible and depended on protein synthesis as they were inhibited by cycloheximide: β-adrenoceptors recovered faster than VIP/helodermin receptors.