Bioavailability and Metabolism of Orange Juice Flavanones in Humans: Impact of a Full-Fat Yogurt

The bioavailability of dietary phytochemicals may be influenced by the food matrix in which they are consumed. In this study the impact of a full-fat yogurt on the bioavailability and metabolism of orange juice flavanones was investigated. Human plasma and urine were collected over a 24 h period after the consumption of 250 mL of orange juice containing a total of 168 μmol of hesperetin-7-O-rutinoside and 12 μmol of naringenin-7-O-rutinoside, with and without 150 mL of full-fat yogurt. The juice also contained 1 g of paracetamol and 5 g of lactulose. HPLC-MS2 analysis revealed the accumulation of hesperetin-7-O-glucuronide, and an unassigned hesperetin-O-glucuronide metabolite in plasma reached a peak concentration (Cmax) of 924 ± 224 nmol/L, 4.4 ± 0.5 h (Tmax) after orange juice ingestion. The Tmax is indicative of absorption in the colon. When the juice was consumed with yogurt, neither the Cmax at 661 ± 170 nmol/L nor the Tmax at 5.1 ± 0.4 h were significantly different from those obtained with juice a...