Determination of 6-Benzylaminopurine and Hg2+ in Bean Sprouts and Drinking Mineral Water by Surface-Enhanced Raman Spectroscopy

A novel method for the determination of 6-benzyladenine (6-BA) in bean sprouts and Hg2+ in drinking mineral water by surface-enhanced Raman spectroscopy (SERS) was described. 6-BA exhibits obvious SERS signal by using the substrate of silver nanoparticles (AgNPs), and the presence of Hg2+ could cause the decrease of SERS signal of 6-BA. The effects of type of aggregation agent, type and level of pH buffer solution, amount of AgNPs, mixing time, concentration of 6-BA, and reaction time on the SERS signals were investigated. Under the optimized experimental conditions, good linear responses were obtained for 6-BA and Hg2+ in the concentration ranges of 10–200 μg L−1 and 5–200 μg L−1, respectively. By the present method, the limits of detection (LODs) for the determination of 6-BA and Hg2+ are 3.3 and 0.20 μg L−1, and the recoveries of 6-BA and Hg2+ in spiked samples are 85.5–113.0 % and 98.2–111.5 %, respectively.