SHG/TPEF-based image technology improves liver fibrosis assessment of minimally sized needle biopsies

Background and aims Sampling size variability of liver biopsy remains a major limitation in the assessment of liver fibrosis. We aimed to evaluate the diagnostic value of a fully quantitative method (second harmonic generation/two-photon excitation fluorescence, SHG/TPEF based) in “short” liver biopsy samples.