Evaluation of the Antifungal Substantivity of Sodium Hypochlorite, Chlorhexidine and MTAD

The purpose of this in vitro study was to compare the antifungal substantivity of 1% MTAD, 2% Chlorhexidine (CHX) and 1.3% sodium hypochlorite (NaOCl) in bovine root dentin. One hundred and ten dentin tubes prepared from bovine incisors were infected in vitro for 72 hours with Candida albicans. The specimens were divided in to five groups as follows: CHX; MTAD; and NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected with round burs into tryptic soy broth (TSB). After culturing the number of colony-forming units (CFU) was counted. In all experimental groups CFU was minimum in the first cultures, and the results obtained were significantly different from each other at any time period (P<0.05). In first culture, the NaOCI group and CHX group showed the lowest and highest number of CFU, respectively. In each group, the number of CFUs increased significantly by time-lapse (P<0.05). In conclusion, the substantivity of MTAD was significantly greater than CHX and NaOCl.