Optimization of industrial-scale high-performance liquid chromatography applications using a newly developed software

Industrial-scale HPLC applications have been evaluated and optimized to give the desired purity and throughput at the lowest total cost in the HPLC step by the use of a newly developed software, including the algorithm Nirvana. The algorithm, capable of handling self-displacement effects, has been utilized to simulate band profiles and yield in the optimization process. It is shown that this method can be used successfully for finding the optimal chromatographic conditions also for applications with self-displacement.