A negative staining technique for high resolution of viruses

The aim of this study is to discuss the possibility of using negative staining techniques on unsectioned material. During investigations concerned with the detailed structure of the protein components of bacteriophage, serious limitations were encountered using existing shadowcasting and sectioning techniques. Fig. 1. Intact T2 phage particles in PTA droplet. Striations along the tail are clearly visible. No staining of the protein is evident