Serodiagnosis of new world Leishmaniasis by using a genus-specific antigen in enzyme linked immunosorbent assays

Abstract A genus-specific monoclonal antibody (83L-5G9), generated against promastigotes of Leishmaniabraziliensispanamensis (WRAIR-470), has been used as a ligand in immunochromatography columns for the recovery and purification of the genus-specific antigen. When the purified polypeptide, actually a doublet comprised of a 58 kd and a 31 kd moiety, was used as the antigen in enzyme linked immunosorbent assays (ELISA), it was reactive with 36 of 85 sera from Leishmania patients and with 1 of 49 sera from confirmed cases of Chagas' disease. An additional 25 sera from an unexposed population and five specimens from individuals seropositive for Toxoplasmagondii were nonreactive.