Silver bromide method as modified for electron microscopic demonstration of ethylenic double bonds existing in ceroid-like pigment bodies.

One of the histochemical characteristics of ceroid pigment is the existence of ethylenic double bonds in protein-bound unsaturated lipids. For electronmicroscopic demonstration of these double bonds, the authors modified the bromine silver method, which was developed by Norton et al. originally for light microscopic demonstration of unsaturated lipids. Using this method, we observed a precipitation of the fine silver grains in highly or moderately electron-dense areas of ceroid-like pigment bodies, evidently showing the presence of double bonds at these sites.