SIMPLE METHOD FOR THE PREPARATION OF I$sup 131$-LABELED INSULIN

Some modifications of the Banerjee technique for the simple and rapid preparation of I/sup 131/-labeled insulin are reported Pig insulin is made to react in buffered solution with sodium metabisulfite and human albumin in the presence of carrier-free I/sup 131/. Electrophoresis at 4 deg C separates the different reaction constituents. The I/sup 131/ has from 0 to 5% decontamination by degradation products, and it has an activity of 100 to 500 mu C/ mu g. (J.S.R.)