Micropropagation of Garden Rose (Rosa Indica L.) through Different Size of Node Explants

The present investigations were carried out to standardize surface sterilization of explants, suitable explants type for culture establishment, growth regulators for shoot multiplication and rooting and to evaluate suitable hardening media. Of the various concentrations of HgCl2 and NaOCl tried for surface disinfection. The explants viz. node were tried. It was observed that complete shoot gave the best results and emerged as suitable explants for Rose culture establishment. Among different concentration of MS medium, ½ MS medium gave early and maximum shoot germination. In all the combination of plant growth regulators, the highest number of shoots regeneration was observed in growth regulator 1.5 mg/l BAP and 1.5mg/l IAA containing medium. The highest shoot initiation was observed in combination MS + 1.5mg/l BAP.