Endogenous reference gene suitable for detection of sesame exogenous gene copy number and building method and application of endogenous reference gene

The invention discloses an endogenous reference gene suitable for detection of sesame exogenous gene copy number and a building method and an application of the endogenous reference gene. The endogenous reference gene is gamma-tocopherol methytransferase gene Se gamma-tmt, wherein a nucleotide sequence is as shown in SEQ ID No:1 or SEQ ID No:2. The invention further discloses the building method of the endogenous reference gene and the application of the endogenous reference gene. The number of exogenous genes in the transgenic plant DNA is detected by a fluorescence real-time quantitative PCR technology, so as to determine the copy number of the endogenous genes in the transgenic sesame plant. The endogenous reference gene suitable for detection of the sesame exogenous gene copy number can be applied to evaluation of a sesame transgenic technology system, large-scale endogenous gene copy number analysis of transgenic sesame, unknown gene copy number evaluation and genetic characteristic analysis of the sesame; a technical foundation is laid for sesame functional genomics research in future and excellent sesame new material evaluation; meanwhile, the operation steps of the copy number detection of the sesame exogenous genes are simple; the result is reliable; and an important basis is provided for obtaining efficient and stable transgenic sesame plants.