Liquid chromatographic determination of glyphosate in water-soluble granular formulations: Collaborative study

A liquid chromatographic (LC) method for the determination of glyphosate in various water-soluble granular formulations was tested by 28 laboratories. Samples were dissolved in mobile phase and injected directly into an LC system with a 25 cm SAX column and a mobile phase of 96% aqueous buffer solution, 0.0062M KH 2 PO 4 , and 4% methanol. Detection was by UV absorption at 195 nm. Manual or automated injections were made via fixed-volume loops. Calculations were based on peak area comparisons with external standards. The collaborative study analyzed 5 matched pairs of 4 water-soluble granular formulations, with one determination per sample. The study generated 138 matched pairs, which were analyzed by using the AOAC spreadsheet and Youden's matched-pair calculations. Coefficients of variation for the 4 formulations ranged from 0.65 to 1.37%. The LC method for the determination of glyphosate in water-soluble granular formulations has been adopted first action by AOAC INTERNATIONAL.