Quadruplex real-time quantitative PCR assay for the detection of pathogens related to late-onset ventilator-associated pneumonia A preliminary report

article i nfo A quadruplex real-time (RT) qPCR assay for the detection and quantification in 4 h of Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii and Stenotrophomonas maltophilia directly from bronch- oalveolar lavage specimens was developed. The specificity of the assay was 100% for all four species.