Isolation and characterization of a temperature-sensitive conditional mutant of Escherichia coli altered for the control of phosphate-regulated proteins

Abstract We have identified a conditional mutation which confers a pleitropic phenotype to Escherichia coli cells: no growth at temperature higher than 36°C, an altered control of the synthesis of several phosphate-regulated polypeptides (including alkaline phosphatase, sn -glycerol-3-phosphate binding protein, phosphate binding protein and outer membrane porin protein PhoE) after growth at 36°C and a wild-type phenotype at 30°C. This mutation was located at minute 89.5 on the E. coli chromosome in a gene we have called cpr for conditional phosphate-regulated.