ANALYSIS OF IMMUNITY IN OSTEOSARCOMA AS A BASIS FOR DEVELOPMENT FOLLOWING THERAPY

Background Osteosarcoma is a malignant bone disease. Predictors which used to determine osteosarcoma from non-invasive (stage IIB) to invasive (stage III), through the ratio of macrophages-1 (M-1) which are pro-inflammatory (anti-cancer) with macrophages-2 (M-2) which have anti-inflammatory characteristic (pro cancer) is not clear yet.  Purpose To elaborate the role of M-1 ratio with M-2 in the development of non-invasive osteosarcoma become invasive, in effort to obtain predictors that can be used to the selection of neoadjuvant therapy time. Methods An observational study with cross sectional analytic study design. The sample of this research was all of paraffin blocks containing osteosarcoma patient tissue, collected within 5 years. The number of samples that met the inclusion criteria was 26 samples, and then divided into two groups, namely group-I as many as 13 stages IIB and group-II as many as 13 samples of stage III. The measured variables are the number of macrophages that express TNF-α and those that express IL-10 by immunohistochemical methods. Results Analysis of the data using a Mann-Whitney test, and the results were obtain is the number of macrophages expressing TNF-α in group-I was greater than in group-II (p <0.05). Likewise, macrophages that expressed IL-10 in group I was smaller than in group-II (p <0.05). Conclusion The M-1 ratio which reflected by TNF-α expression cells with M-2 as reflected by IL-10 expression cells in the non-invasive group is 5 1.Whereas in the group that develops to invasive is 1 6.