Enzyme linked immunosorbent assay (ELISA) kit for detecting sulfamethazine and enrofloxacin and application thereof

The present invention discloses an ELISA kit for detecting sulfamethazine and/or enrofloxacin and application thereof. The kit provided by the present invention includes an antibody 1, an antibody 2, a coating antigen 1, a coating antigen 2, an enzyme marker 1 and an enzyme marker 2. The antibody 1 is an anti-sulfamethazine antibody, the antibody 2 is an anti-enrofloxacin antibody, the coating antigen 1 is a conjugate of sulfamethazine hapten and a carrier protein, the coating antigen 2 is a conjugate of enrofloxacin hapten and a carrier protein, the enzyme marker 1 is an enzyme-labeled second antibody resisting the antibody 1, and the enzyme marker 2 is an enzyme-labeled second antibody resisting the antibody 2. The kit provided by the present invention can qualitatively or quantitatively detect sulfadimidine and enrofloxacin residue in a sample to-be-detected. Sample pretreatment process is simple. The detection method of the invention is simple, and high in specificity, sensitivity and accuracy.