Simultaneous Determination of Geniposide and Baicalin Content in Xiongdan Pills by Double Wavelength HPLC

OBJECTIVE To establish a double wavelength HPLC for simultaneously determination of geniposide and baicalin in Xiongdan pills.METHODS The analysis was performed on an Hibar C18 column(150 mm×4.6 mm,5 μm) with gradient elution using 0.2% phosphoric acid(A) and acetoniterile(B) in the order of B 15%(0-9 min),15%→25%(9-10 min),25%(10-17 min),the flow rate was 1.0 mL·min-1.Geniposide and baicalin were detected at 240 nm and 278 nm,respectively.The column temperature was room temperature.RESULTS The calibration curves of geniposide and baicalin showed good linearity in the ranges of 0.08-0.8 μg and 0.23-2.3 μg.The average recoveries were 96.0% and 103.6% with RSD of 1.75% and 2.01%.CONCLUSION The method is simple,accurate and reproducible for simultaneous determining geniposide and baicalin in Xiongdan Pills.