Nanoparticle Aggregate‐Based Fluorescence Enhancement for Highly Sensitive and Reproducible Detection of DNA
2014
Sensitive detection of DNA at the sub picomolar range is demonstrated using a magnetic bead sandwich hybridization assay coupled with surface-enhanced fluorescence (SEF)-based amplification. Unlike enzymatic amplification, the SEF amplification step does not add any additional background to the measurement and does not cause a significant increase in the coefficient of variance (CV).
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