Induction and analysis of synchronous meiotic yeast cultures

Meiosis in Saccharomyces cerevisiae can be induced by deprivation of nutrients. Here, we present a protocol for inducing synchronous meiosis in SK1, the most efficient and synchronous yeast strain for meiosis, by exposing SK1 cells to liquid medium that contains potassium acetate as a nonfermentable carbonsourceandlacks nitrogen.These synchronousmeiotic yeastculturescanbesubjected toarange of molecular and cytological analyses, making them useful for investigating the genetic and molecular determinants of meiosis.