Establishment and identification of in vitro HL7702 and HepG2 cell ines with HBX gene expression.

Aim To establish in vitro cell line with stable and effective HBxAg expression,for study of carcinogenesis mechanism of HBX gene and HBxAg.Methods A recombinant plasmid (pcDNA3.1-HBX)containing the full length of HBX gene was transfected into HL7702 cells and HepG2 cells.The expression of HBx in the cells was analyzed by Western blot and immunofluorescency using anti-HBx antibodies.Results Cell clones were obtained after being transfected with pcDNA3.1-HBX plasmid.Stable expression of HBxAg was detected by Western blot,immunofluorescency and RT-PCR.Conclusion HBX gene expression cell lines were constructed in this study.The property of this system ensures stable and high level expression of HBx.It is a useful tool for functional studies of HBX gene.