Evaluation of HER2 gene amplification status in invasivebreast cancer patients by Fluorescence in Situ Hybridizationanalysis and its correlation with clinical features
2015
Precise assessment of HER2 gene status as an important biomarker plays a
significant role in identifying the eligible patients for Trastuzumab therapy
and determining their clinical outcomes. In this study, the researchers
assigned HER2 amplification status in invasive breast cancer specimens by
Fluorescence in Situ Hybridization (FISH) and determined its association
with other clinical features. Formalin-fixed paraffin embedded tumor tissue
specimens of 46 patients with invasive breast cancer were collected from
November 2011 till May 2012. HER2status was evaluated by FISH. The
Zytolight SPEC HER2/CEN17 dual color probe kit was applied for
assessment of HER2status. HER2 gene amplification was defined as
HER2/CEP17 ratio>2.2.The association between HER2status and clinical
features like tumor grade, tumor type, tumor size, axillary lymph node
involvement and age of patient was done using Chi squared test at the 0.05
level of significance (p value). Amplification of HER2 gene was detected
in twelve cases (26%). On statistical analysis HER2status showed
correlation with tumor grade (p=0.02).There was no correlation between
HER2status and tumor type, tumor size, lymph node status and age of
patients. The results of this study are consonant with the findings of other
studies about the presence of HER2 gene amplification in invasive breast
cancer. Statistical analysis showed patients with HER2 amplified gene
have tumors with higher grade. In these patients the probability of
increased proliferation and metastasis is high therefore evaluation of HER2
gene amplification status in breast cancer patients specially in high grade
tumor with an accurate method such as FISH is essential
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