Three-Dimensional Geometry of Honeycomb Collagen Promotes Higher Beating Rate of Myocardial Cells in Culture

Myocardial cells were isolated from newborn rats, cultured on a novel three-dimensional (3-D) honey- comb collagen scaffold (HC) and their morphology and beating rates compared with ones on conventional plastic dishes.On thefirst day,the cells attached to HC had already started beating. As time went on, the rate of beating increased as the pores of HC gradually filled with the cells, which integrated to form the cell-matrix complex.At day 8, beating reached the highest frequency of 162 beats per minute, which was twice that of the control cells on plastic dishes. It was concluded that 3-D geometry of the HC is conducive to functional growth of the myocardial tissues, and will potentially be useful for tissue engineering of myo- cardial regeneration. Key Words: Honeycomb collagen —Myocardial cells—Three-dimensional culture—Biocom- patibility—Beating rate.