HIV-1 envelope glycoprotein characteristics that correlate with the development of cross-reactive neutralizing activity in HIV-1 infected individuals

Results For 31 out of 34 patients we observed a correlation between a higher level of CrNA and on one hand a shorter variable region 1 (P=0.04) and on the other hand a increased number of NXS sequons relative to the number of PNGS (P=0.04), which decreases the probability of glycosylation at that site. In contrast, in the 3 patients with the most potent CrNA, defined as elite neutralizers, the viral V1 region was longer with a higher number of NXT sequons relative to the number of PNGS. These viral characteristics are similar to those in patients with low potency of CrNA, rather than to those in patients with higher potency of CrNA in their serum. Conclusion Our results suggest that in general the development of CrNA in HIV-1 infected patients is associated with a more open structure of the viral Env, mediated by a short V1 loop and a low probability of glycosylation. However, our three elite neutralizers were exceptions to this rule. The identification and understanding of Env characteristics that are involved in the development of CrNA should help the rational design of an effective antibody-based vaccine immunogen.