A new “turn-on” fluorescence probe based on hydrazine-triggered tandem reaction

Abstract Hydrazine is extremely harmful to the human body. The leakage of hydrazine is liable to cause potential safety hazards. Here, we reported a new fluorescence probe based on the tandem reaction. The hydrazine-triggered hydrazinolysis-cyclization resulted in the formation of the iminocoumarin. The fluorescence intensity at 522 nm of the probe increased after the reaction with hydrazine. There was a linear relationship between the fluorescence intensity and the concentration of hydrazine (0.14–120.00 μM). The LOD of the probe to N2H4 was 1.36 ppb. Notably, the probe could detect hydrazine in BT474 cells and tap water.