Fast volumetric calcium imaging across multiple cortical layers using sculpted light

Robert Prevedel,Aart J. Verhoef,Alejandro J Pernía-Andrade,Siegfried Weisenburger,Ben S. Huang,Tobias Nöbauer,Alma Fernández,Jeroen E Delcour,Peyman Golshani,Andrius Baltuska,Alipasha Vaziri

Fast volumetric calcium imaging across multiple cortical layers using sculpted light

2016

Robert Prevedel
Aart J. Verhoef
Alejandro J Pernía-Andrade
Siegfried Weisenburger
Ben S. Huang
Tobias Nöbauer
Alma Fernández
Jeroen E Delcour
Peyman Golshani
Andrius Baltuska
Alipasha Vaziri

Two-photon scanning microscopy is inherently slow and thus limits volumetric calcium imaging. Prevedel et al. achieve increased volumetric imaging speed by tailoring the excitation volume via light sculpting.

Keywords:

Molecular biology
Multiphoton fluorescence microscope
Microscopy
Analytical chemistry
Calcium imaging
Biology
Fluorescence-lifetime imaging microscopy
volumetric imaging
ca2 imaging
Biomedical engineering
scanning microscopy
Molecular imaging

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