Immune Analysis on mtHSV Mediated Tumor Therapy in HSV-1 Seropositive Mice

We previously reported that the mutant virus of HSV (mtHSV) mediated tumor therapy was efficacious in Balb/c and nude mice. However, it is significant to know whether mtHSV works in HSV-1 tumor seropositive individuals because many patients with HSV-1 seropositivity have been found in clinical trial. Here we asked whether the oncolytic effect of mtHSV is influenced under condition of HSV-1 seropositivity. In this study, Balb/c mice were immunized intraperitoneally with mtHSV and HSV-1 F. Subsequently, HSV seropositive and naive Balb/c mice were inoculated with sarcoma 180 cells in the armpit of left forelimb and then were treated with mtHSV intratumorally. The results shown that the growth of tumor in HSV-1 F seropositive mice was inhibited dramatically after treatment with mtHSV; and significant differences in mean tumor growth ratio were found compared with the groups of mtHSV seropositive mice and naive mice. These data demonstrated that the effect of mtHSV-mediated tumor therapy couldn’t be weaken but...