Development of an Efficient Tissue Culture Protocol from Different Explants of Spilanthes Acmella M.

The present study was conducted to investigate the effects of different explants, different concentrations and combinations of growth regulators on callus induction and plant regeneration of Spilanthes acmella M. Leaf explants were inoculated onto MS medium supplemented with different concentrations of α -naphthalene acetic acid (NAA), 2,4-Dichlorophenoxy acetic acid (2,4-D), Indol acetic acid (IAA) and benzyl adenine (BA) alone and auxins in combinations with BA for callus ­­­­­ induction. Callus initiation was observed in all media evaluated and the highest callus formation (0.29 ± 0.03 mg) wasobtained from explants cultivated in the presence of IAA alone at 10 µM in nodal explant or 15 µM BA + 5µM 2,4-D (0.33 ± 0.039 mg)in combination.Maximum shoot induction (81%) and highest number of shoot per call was obtained from callus induced on 15 µM BA + 5µM 2,4-D3 weeks after transferring the callus to a MS medium supplemented 1 0 µM BA + 5 µM NAA.The regenerated plantlets were well rooted by exposing to auxins (NAA, IAA, IBA) and auxin in combinations with increased level of sucrose. The successof plant tissue culture for in vitro culture of S.acmella was encouraged by acclimatization of the plantlets in the greenhouse conditions.