维甲酸对人神经母细胞瘤SK-N-SH细胞形态结构和分化标志物表达的影响

Objective: To investigate the biological effects of retinoic acid OH the cell morphology and terminal differentiation of the human neuroblastoma SK-N-SH cells. Methods: After treated with 1μmol/L retinoic acid, SK-N-SH cells were subjected to light-microscopy, electro-microscopy and immuncocytochemical assay, and were compared with the untreated cell. Results: The results of light and electro-microscopy observation showed that the morphology and ultrastructure of SK-N-SH cells had changed after treatment. Cells became multipolar with several neuritis: cell bodies became smaller with long axonal processes between one another to form ganglion; less microvilli in the cell faces; the nucleolus reduced while the euchromatin increased: and the organelles developed. Immuacocytochemical analysis revealed that the expression of the neuronal markers of terminal differentiation, including NSE, MAP2 and Synaptophysin, were strongly positive after treatment. Conclusions: Retinoic acid could reverse the malignant phenotypes of SK-N-SH cells and promote the expression of terminal differentiation biomarkers, thus playing a significant role in cell inducement into terminal differentiation.