Microprapogation of callus from Syzygium cumini explant

Microprapogation of the syzygium cumini explant was tried by using the different kind of MS. medium, hormonal changes in the medium show variati ons in the result. At the appropriate quantity of the hormones the explant shows a significant amo unt of development; the basal medium does show the some kind of development into a bud & no effect on the leaf explant development. The auxin is generally root initiator and cytokinin are the shoo t developers. Very low level of growth regulator concentration does not show the significant result. (0.1-mg/l -0.1 mg/l) Present work we got development of callus by using a MS medium of basal medium + 6 BAP with additional supplement of IBA and kinetin at concentration (0.60 mg/l ‐ 0. 60 mg/l) and at the (0.02-0.5 mg/l) of IBA & kinetin shoot development observed. IAA only shows some growth in explant but does not develop the tumor/ callus.