Expression of VEGF and PCNA in bladder transitional cell carcinoma and their clinical significances

Objective:To study the expression of VEGF and PCNA in bladder transitional cell carcinoma(BTCC) tissue and the relationship between these expression and clinicopathological parameters,in order to understand the role of VEGF and PCNA in the angiogenesis of tumor and tumor cell proliferation.Methods:The expression of VEGF nad PCNA in 77 cases of the BTCC were detected by immunohistochemical LDP methods.Results:The positive expression rates of VEGF and PCNA in 77 cases of BTCC were 66.23%、51.94%,respectively.Both the expression of VEGF and PCNA were related to the tumor pathologic grade and the invasiveness of tumor(P0.05).The PCNA-positive cells(expression) in VEGF-positive tumor were significantly higher than those in VEGF-negative tumor(P0.01).In addition,PCNA-positive cells(expression) significantly increased with increasing VEGF expression(P0.05).The positive correlation was noted between VEGF expression and PCNA expression(P0.05).Tumor with high VEGF that were positive for PCNA recurred earlier and had a greater risk of stage progression than did those low VEGF and PCNA negative tumors.The high levels of VEGF also indicated the poorest prognosis in PCNA negative than did those the low VEGF tumors.Conclusions:This study indicates that VEGF and PCNA is closely related the histopathological grading,the tumor differentiation and prognosis of BTCC.The pathological changes in the tumor is typically vessel dependency and cell proliferation.VEGF and PCNA may be involved in the regulation to tumor angiogenesis and tumor cell proliferation through the different ways.