Tolerance to simulated ice encasement and Microdochium nivale in USA selections of greens-type Poa annua

Abstract Lack of winter hardiness is a limitation to the use of annual meadowgrass (Poa annua f. reptans) on golf course putting greens in northern climates. Our objectives were 1) to assess the tolerance of 13 selections of annual meadowgrass to simulated ice encasement (SIE) and pink snow mould (PSM, Microdochium nivale); 2) to determine if these tolerances were interrelated or related to plant concentrations of water soluble carbohydrates (WSC); and 3) to determine if tolerance to SIE can be associated with accumulation of toxic substances. Samples of the annual meadowgrass selections were taken from an experimental green at University Park, Pennsylvania, on four dates from 23 Nov. 2005 until 27 Mar. 2006. Samples of creeping bentgrass (Agrostis stolonifera L.) ‘Penn A-4’ were included for comparison in studies on SIE and WSC. Samples were vacuum-sealed in plastic bags at 1°C for SIE and inoculated with three isolates of M. nivale for PSM tolerance. Small, but significant, differences were found among ...