Influence of Huangqi Injection on vascular endothelial injury in multiple organ dysfunction syndrome after anabiosis

Objective To investigate the influence of Huangqi Injection on vascular endothelial injury in multiple organ dysfunction syndrome(MODS) after anabiosis with different resuscitation fluid through establishing the model of MODS in rats with two hits.Methods SD rats(n=32) were randomly divided into control group,RL group(resuscitated with Lactated Ringer's Solution),HTH group(resuscitated with 7.5% hypertonic saline and 6% hydroxyethyl starch mixture),and HTH group was divided again into HTH1 group(without Huangqi Injection after anabiosis) and HTH2 group(with Huangqi Injection after anabiosis).The animal model of MODS after anabiosis was copied in RL group and HTH group.HTH2 group was given intraperitoneal Huangqi Injection and HTH1 group and RL group was given 0.9% sodium chloride solution.After 24 hours the blood samples and lung tissue samples were collected for detecting the number of serum circulating endothelial cells(CEC),and content of nitric oxide(NO),endothelin-1(ET-1),lung tissue tumor necrosis factor-α(TNF-α) and intercellular adhesion molecule-1(ICAM-1),interleukin-6(IL-6),vascular cell adhesion molecule-1(VCAM-1) mRNA,and pathological changes of lung tissue were examined with light microscope.Results The number of CEC and content of NO and ET-1 were significantly lower in HTH2 group than those in RL group and HTH1 group(all P0.05).The content of TNF-α,ICAM-1,IL-6 and VCAM-1 were significantly lower in HTH2 group than those in HTH1 group(all P0.05).Conclusion Huangqi Injection has obvious protective effect on vascular endothelial of MODS after anabiosis with different resuscitation fluid.