Simultaneous microdetermination of pyrazinamide and pyrazinoic acid by liquid chromatography

: The technique reported here enables to titrate concomitantly pyrazinamide as well as its main metabolite, the 2-pyrazinoic acid. The serum sample is deproteinized with acetonitrile and the portion floating on the surface is injected on an anionic column of copolymer. The detection occurs at 270 nm and the sensitivity of the method enables its use during pharmacokinetic studies. The other anti-tuberculous drugs, usually associated, produce no interference.