Cloning and Characterization of β(1,3;1,4) Beta Glucanase Gene from TcLr35 Wheat

One pair of primer was designed based on the β(1,3; 1,4) beta glucanase,a full length wheat β(1,3; 1,4) beta glucanase gene was obtained by RT-PCR and RACE from the RNA of TcLr35 carrying the Lr35 gene conferring resistance against wheat leaf rust induced by the Puccinia triticina named PR34,which was 1 416 bp in length, and included one complete open reading frame encoding 335 amino acids,a 379 bp 3' untranslated region(3' UTR) and 29 bp poly(A) tails. The initiation codon and stop codon were found in 13 bp locus and 1 015 bp locus respectively. The deduced amino acid sequence showed close homology to beta glucanase proteins which have been isolated from wheat, barley,and many other plants. The deduced amino acids contained Glyco_hydro_17 conserved domains which was related to beta glucanase. Southern blot indicated that the TcLr35 genome contained low copies of PR34 gene.