Defense of the clone: antibody 259D effectively labels human FOXP3 in a variety of applications

To the editor: Three related articles in Blood have recently highlighted an issue with a significant impact on the study of regulatory T cells (Tregs), namely the specificity and affinity of the antibodies used to detect forkhead box P3 (FOXP3).[1][1][⇓][2]–[3][3] There is phenomenal interest