Shell controllable silk fibroin micro-capsules and preparing method thereof

The invention discloses shell controllable silk fibroin micro-capsules and a preparing method thereof. The preparing method comprises the steps of 1, mixing silk fibroin, template particles and carbonate buffer solution to obtain first mixed liquor, adding a poor solvent of protein, conducting mixing and centrifugation, removing supernate, and conducting washing to obtain first particles; 2, mixing the first particles, silk fibroin and carbonate buffer solution to obtain second mixed liquor, and conducting centrifugation, supernate removal and washing to obtain second particles; 3, repeating step 2; 4, adding glutaraldehyde aqueous solution to particles obtained from step 3 for protein crosslinking, and conducting centrifugation and washing to obtain final particles; removing the template particles, and then conducting washing to obtain shell controllable silk fibroin micro-capsule suspension. The micro-capsules are hollow and can load biomacromolecule drugs or other functional components. Operation is easy, time and energy are saved, the method is suitable for large-scale production, and adopted materials have high biocompatibility and biodegradability and are free of immunogenicity.