Antibody separation methods, the antibody evaluation methods, evaluation methods of the pharmaceutical and a kit for 2D electrophoresis of antibody

Antibodies separation method for separating an antibody while maintaining the disulfide bond, the first dimension which separates the antibody protein dissolved in prepared buffer free of reducing agent, the first dimension electrophoresis using a buffer containing no reducing agent includes an electrophoretic process, the antibodies proteins electrophoresed in the first dimension electrophoresis step, a second dimension electrophoresis separating by second dimensional electrophoresis using a buffer containing no reducing agent