[MiR-150-5p inhibits the proliferation and promoted apoptosis of pancreatic cancer cells].

Objective To investigate the role of miR-150-5p in cell proliferation and apoptosis in human pancreatic cancer cell lines.Methods The expression of miR-150-5p in pancreatic cancer was detected by real time qPCR analysis in 11 pairs of pancreatic cancer tissue and matched adjacent normal tissue samples and in 4 pancreatic cancer cell lines.PANC-1,MIA PaCa-2,BxPC-3 and AsPC-1 cells were transfected with chemically synthesized MiR-150-5p mimics,and CCK-8 assays was then performed to assess cellular functions.To fully understand the mechanisms by which miR-150-5p exerted its function,cell cycle analysis was performed on MIA PaCa-2 and PANC-1 cells 48 hours after transfection,by incubating with propidium iodide (PI)and subsequently analyzed by fluorescence-activated cell sorting (FACS).Apoptosis assay was performed on MIA PaCa-2 and PANC-1 cell lines 24 hours after transfection using the Annexin V-FITC Apoptosis Detection Kit I (BD Biosciences) and analyzed by FACS.Results The expression of miR-150-5p was consistently lower in the pancreatic cancer tissues than in normal tissues,and the miR-150-5p was also down-regulated in pancreatic cancer cell lines (P ＜ 0.05).MiR-150-5p mimics transfection siguificantly raised the expression level of miR-150-5p mRNA in PANC-1 and MIA PaCa-2 (P ＜0.01).The CCK-8 proliferation assay showed that cell growth was reduced in 4 pancreatic cancer cell lines (AsPC-1,BxPC-3,MIA PaCa-2,PANC-1) of miR-150-5p transfected cells compared with NC-transfected cells.The inhibition rates were 50.7％,48.6％,30.8％ and 42.3％,respectively (P ＜ 0.01).The apoptotic rate was increased in cells transfected with miR-150-5p mimics (P ＜0.01).The cell cycle analysis in MIA PaCa-2 indicated that miR-150-5p treatment induced cell cycle arrest in G1 phase with a significant increase in the percentage of cells in G1 phase (P ＜ 0.01),and a reduction of the S-phase cell population in MIA PaCa-2 and PANC-1 (P＜0.01).Conclusions MiR-150-5p is down-regulated in pancreatic cancer.Overexpression of miR-150-5p inhibits cell proliferation,blocked the cell cycle,but promotes cell apoptosis in pancreatic cancer cells. Key words: Pancreatic neoplasms ;  MicroRNAs;  Tumor cells,cultured