Cloning and Characterization of a Fructose-1,6-bisphosphate Aldolase Gene in Medicago sativa L.

Based on an EST of Medicago sativa L.,a full length of 1 487 bp cDNA was isolated using SMART RACE.This gene was named MsALD(GenBank accession No.FJ896113).The sequence analysis has revealed that MsALD contains a maximum open reading frame of 1 194 bp for 398 amino acids.The putative amino acid sequence was highly similar to ALD from some other plants,such as Trifolium pratense,Solanum tuberosum and Nicotiana paniculata.Sequence analysis indicated that MsALD belongs to the class I ALD.The result of the RT-PCR showed that MsALD is highly expressed in Medicago sativa L.after being treated for 2 hours.This indicated that MsALD may play an important role in salt tolerace of Medicago sativa L..