[Regulation of MAVS-mediated pathway by p21].

AIM: To study the interaction between MAVS and p21 regulates MAVS-mediated pathway.METHODS: Firstly,we cloned full-length p21 gene by PCR.And then the interaction between MAVS and p21 was further demonstrated by co-immunoprecipitation.The regulation of MAVS-mediated pathway by p21 was analyzed by luciferase reporter assays.RESULTS: There are interaction between p21 and MAVS in 293T cells.p21 downregulates activity of MAVS.CONCLUSION: It is shown that p21 inhibits MAVS-mediated activation of NF-κB and IFN-β by Luciferase reporter assays.Overall,these results laid the foundation for further investigating the function of p21 in MAVS-mediated pathway.