Development of an immunocompetent human skin tissue model using three dimensional (3D) freeform fabrication

We report on the development of a novel in vitro, multi-compartmental, three-dimensional, Human Skin Tissue Experimental Model (HISTEM) using three-dimensional free form fabrication (3DFFF). The availability of this model is expected to positively impact the development of transdermal vaccines and immunomodulators for infectious diseases and autoimmune disorders. Our studies demonstrate the biological and structural equivalence of HISTEM with ex vivo human skin.