Hn protein of avian paramyxovirus-4 and the diagnostic composition for paramyxovirus-4 containing the same

PURPOSE: HN protein of avian paramyxovirus-4 (APMV-4) and an antigen diagnostic solution for HI method examination including the same are provided to be able to exclude the danger of neighbor contamination or disease contagion in the process of treating live APMV-4, and to be able to mass-produce the APMV-4 antigen and antibody diagnostic solution in a rapid and economic method. CONSTITUTION: A manufacturing method of an expression vector of recombinant baculovirus including HN gene of APMV-4 comprises (1) a step of extracting virus genome RNA from APMV-4 La Sota strain; (2) a step of synthesizing cDNA of HN protein gene of APMV-4 by using the extracted RNA and reverse primer of Sequence 2; (3) a step of amplifying the synthesized cDNA, forward primer of Sequence 1, and reverse primer of Sequence 2 by using PCR, and synthesizing DNA; (4) a step of extracting DNA fragment by treating the synthesized DNA with EcoR1 and Hind III; and (5) a step of inserting the extracted DNA fragment into the multicloning site of pFastBac^TM1 expression vector having baculovirus polyhedrin promoter (PPH). The forward primer of Sequence 1 is that EcoR1 restriction enzyme acting site is inserted into the upper part of ATG of 5' end terminal. The revers primer of Sequence 2 is that Hind III restriction enzyme acting site is inserted into the upper part of end codon of 5' end terminal.