Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools.

We have previously reported that exposure of endothelial cells to cyclic strain elicited a rapid but transient generation of inositol 1,4,5-trisphosphate (IP 3 ), which reached a peak 10 s after the initiation of cyclic deformation. To address the effect of cyclic strain on intracellular Ca 2+ concentration ([Ca 2+ ] i ) and its temporal relationship to IP 3 generation, confluent bovine aortic endothelial cells were grown on flexible membranes, loaded with aequorin and the membranes placed in a custom-designed flow-through chamber. The chamber was housed inside a photomultiplier tube, and vacuum was utilized to deform the membranes. Our results indicate that the initiation of 10% average strain induced a rapid increase in [Ca 2+ ] i which contained two distinct components: a large initial peak 12 s after the initiation of stretch which closely followed the IP 3 peak, and a subsequent lower but sustained phase. Pretreatment with 5  μ MGdCl 3 for 10 min or nominally Ca 2+ -free medium (CFM) for 3 min reduced the magnitude of the initial rise and abolished the sustained phase. Repetitive 10% average strain at a frequency of 60 cycles/min also elicited a single IP 3 peak at 10 s. However, there was also a large initial [Ca 2+ ] i peak followed by multiple smaller transient [Ca 2+ ] i elevations. Preincubation with 5  μ MGdCl 3 or CFM diminished the initial [Ca 2+ ] i transient and markedly inhibited the late-phase component. Preincubation with 25  μ M 2,5-di-( t -butyl)-1,4-benzohydroquinone (BHQ) attenuated the initial [Ca 2+ ] i transient. Cyclic-strain-mediated IP 3 formation in confluent endothelial cells at 10 s, however, was not modified by pretreatment with 25  μ M BHQ, 500  μ M NiCl 2 , 10 nM charybdotoxin, 5  μ M GdCl 3 or CFM. We conclude that in endothelial cells exposed to cyclic strain, Ca 2+ enters the cytosol from intracellular and extracellular pools but IP 3 formation is not dependent on Ca 2+ entry via the plasma membrane.