Abstract 15630: Novel Mitochondrial Protein D-glutamate Cyclase Decreases During the Progression of Heart Failure

Introduction: Recently, importance of D-amino acids in mammals has been drawing attention. D-Amino acids are enantiomers of L-amino acids and have been recognized as biomarkers and bioactive substances in mammals, including humans. However, functions of D- amino acids in the heart are still unclear. Methods and Results: We compared mitochondrial protein and gene expression between transverse aortic constriction (TAC)-operated mouse hearts and sham-operated mouse hearts using with isobaric Tags for Relative and Absolute Quantitation (iTRAQ) and microarray. Among these, expression of 9030617O03Rik, which has not been functionally characterized, was decreased in heart failure. The decrease of 9030617O03Rik was confirmed by real-time PCR and western blotting. In addition, we found that this protein is also reduced in human heart failure. Further analyses showed the protein is rich in heart, kidney, and present in the internal matrix membrane of mitochondria. Protein structure predictions showed that 9030617O0...